Structural characterization and antifungal activity of Algerian Opuntia ficus-indica polysaccharides: FTIR, GC-MS, colorimetric assays, and bioactivity dataStructural and antifungal data: Sequentially extracted polysaccharides from Algerian Opuntia ficus-indica cladodes (FTIR, GC, colorimetric assays)

This dataset comprises raw and processed Fourier-transform infrared (FTIR) spectra obtained via attenuated total reflectance (ATR) for six polysaccharide fractions extracted from Algerian <em>Opuntia ficus-indica</em> cladodes: <strong>Mucilage 1 &amp; 2</strong> (water-soluble fractions) <strong>Pectin 1 &amp; 2</strong> (hot water/EDTA-extracted) <strong>Hemicellulose</strong> (alkali-extracted) <strong>Cellulose</strong> (residual fraction) <strong>Crude extract</strong> (unfractionated control) <strong>Key Features:</strong> <strong>- Spectral Range</strong>: 4000–400 cm⁻¹ at 4 cm⁻¹ resolution (32 scans)<br> - <strong>Identified Functional Groups</strong>: O–H stretching (3400 cm⁻¹) Carboxylate C=O (1750 cm⁻¹, pectins) β-glycosidic linkages (890 cm⁻¹)<br> - <strong>Correlative Data</strong>: Monosaccharide composition (GC) and antifungal activity against <em>Penicillium sp.</em> are linked to spectral peaks. <strong>Potential Applications:</strong> Validation of plant polysaccharide extraction protocols Structure-activity studies for natural antifungals Comparative analysis of xerophytic plant adaptations <strong>Technical Details:</strong> <strong>Files Included</strong>:<br> FTIR-DRAOU.xlsx spectra (wavenumber vs absorbance) <strong>Instrument</strong>: Thermo Scientific FTIR spectrometer (OMNIC™ Software) <strong>Ethics</strong>: No human/animal subjects involved. <br> This dataset supports the research article "<em>*Structural characterization and antifungal properties of...*</em>" [DOI/Lien manuscrit si disponible]. It contains: <br> <br> 1. <strong>FTIR-ATR Spectra</strong> <br> - Raw spectra (4000-400 cm⁻¹) of mucilage (M1-M3), pectins (P1-P2), hemicelluloses, and cellulose fractions. <br> - Key peaks: 1750 cm⁻¹ (C=O), 890 cm⁻¹ (β-glycosidic linkages). <br> <br> 2. <strong>Gas Chromatography (GC)</strong> <br> - Monosaccharide composition (% molar) of all fractions. <br> - Includes arabinose, rhamnose, xylose, galacturonic acid quantifications. <br> <br> 3. <strong>Colorimetric Assays</strong> <br> - Neutral sugars (Dubois method) and uronic acids (Blumenkrantz method). <br> - Corrected values using Montreuil-Spik equations. <br> <br> 4. <strong>Antifungal Bioassays</strong> <br> - <em>*Penicillium*</em> sp. growth inhibition data (dose-response, IC50). <br> <br> <strong>Methods</strong>: <br> - Extraction: Sequential water/EDTA/KOH protocol. <br> - GC: Shimadzu GC-2030 (DB-5 column, TMS derivatization). <br> - FTIR: Nicolet iS50, 4 cm⁻¹ resolution. <br> <br> <strong>Usage Notes</strong>: <br> - Negative uronic acid values in cellulose set to 0. <br> - Missing data = "ND" (Not Detected).