Neonatal overnutrition perturbs glucose homeostasis and causes hepatic steatosis in diet-induced obesity.

A-B: Glucose and (A) insulin (B) tolerance tests and incremental area under the curves/inverted incremental area under the curves of adult SL and NL mice fed a chow or a high-fat diet (HFD) (n = 4–10 per group from ≥ 4 litters). C-E: Plasma levels of glucose (C), insulin (D), and hepatic triglyceride content (E) of adult SL and NL mice fed a chow or a HFD (n = 5–11 per group from ≥ 5 litters). F: Representative images showing Oil Red-O stain in the liver of adult SL and NL mice fed a chow or a HFD. *P<0.05, **P<0.01 and ***P<0.001 versus NL matched for diet; ΔΔ P<0.01 for diet main-effect. Scale bar, 100 μm.