Genetic variation and clonal diversity in introduced populations of Mimulus guttatus assessed by genotyping at 62 single nucleotide polymorphism loci

Background: Single nucleotide polymorphisms (SNPs) are increasingly being used to study non-native populations. SNPs are relatively information poor on a per locus basis, but allow genotyping more loci than others markers (e.g., microsatellites) and have the advantage of consistent allele calls between studies. Aims: We investigated the utility of a newly developed set of SNP markers, suitable for high throughput genotyping to characterise genotypic variation and population structure in non-native populations of the facultative clonal herb Mimulus guttatus in the United Kingdom (UK). Methods: We analysed 62 SNP markers and using a high throughput platform genotyped 383 individuals from 10 populations from the native range in North America and 14 populations in the UK. Results: We found wide variation in genotypic diversity within UK populations, indicating reproductive strategies that vary from mostly clonal to mostly sexual. All but one UK population were, on average, more clo...背景：单核苷酸多态性（single nucleotide polymorphisms, SNPs）正日益广泛地应用于非本土种群的相关研究。单核苷酸多态性在单个位点上的信息含量相对有限，但相较于微卫星等其他分子标记，可实现更多位点的基因分型，且具备不同研究间等位基因分型结果一致性高的优势。 研究目的：本研究评估了一套新开发的、适用于高通量基因分型的SNP标记集的应用价值，旨在表征英国（UK）境内兼性克隆草本植物沟酸浆（Mimulus guttatus）非本土种群的基因型变异与种群结构。 方法：我们选取62个SNP标记，借助高通量分型平台，对来自北美原生分布区的10个种群以及英国境内14个种群的383个个体进行了基因分型。 结果：我们在英国种群中检测到广泛的基因型多样性差异，表明其繁殖策略从近乎完全克隆到近乎有性繁殖不等。除一个英国种群外，其余所有英国种群的平均……