Genome-wide maps of Cic binding sites in intestinal stem cells (ISCs) & Entroblasts (EBs) and transcriptome profiling of Cic depleted progenitor cells (ISCs & Ebs).

Purpose: Cic is a transcription factor regulating intestinal stem cell (ISC) proliferation as downstream effector of EGFR signaling pathway. ISC-specific mRNA expression profiling and DNA binding mapping using DamID were performed to identify potential target of Cic.Methods: Total RNA was isolated from GFP+ FACS sorted cells and sent for sequencing after amplification.Conclusions: We identified potential target genes of Cic, which is differetially expressed in Cic depleted ISCs and also associated Cic binding peaks.