Identification of nucleolar associated domains (NADS). Identification of nucleolar associated domains (NADS)

This study aims to identify genomic contacts with the nucleolus (nucleolar associated domains, NADs). We established a novel methodology that we applied to identify NADs in embryonic stem cells (ESCs). The results revealed unprecedented distinct layers of genome compartmentalization that are distinguished according to gene expression and chromatin states thereby providing novel insights into basic principles of genome organization and its role in gene expression and cell function. Overall design: Nucleolar-DamID, ChIP seq, RNA seq in mouse ESC