Figure S1 - Targeting Gastrin-Releasing Peptide Suppresses Neuroblastoma Progression via Upregulation of PTEN Signaling

Doxycycline treatment induced GRP silencing in neuroblastoma cell lines. (A) Semi-quantitative analysis of GRP mRNA levels confirmed silencing after doxycycline treatment for 48 h in BE (2)-C/Tet/shGRP and SH-SY-5Y/Tet/shGRP. (B) Real-time PCR demonstrated significant silencing of GRP mRNA levels after doxycycline treatment for 48 h in BE (2)-C/Tet/shGRP and SH-SY5Y/Tet/shGRP (mean ± SEM; * = p (EPS)