Global Transcription Profiling and Virulence Potential of Streptococcus pneumoniae after Serial Passage. Streptococcus pneumoniae

Alterations in global transcriptional profiles of S. pneumoniae 6304 serotype 4 after 50 passages (50P) and 100 passages (100P) on laboratory media Keywords: Adaptation to unique growth environment Overall design: Three conditions experiment: Control, single passage strain (1P), 2 biological replicates Experimental 50 passage strain (50P), 2 biological replicates Experimental 100 passage strain (100P), 2 biological replicates All strains independently grown and harvested at OD600 ~ 0.5. Four spot replicates per array.