Genome sequencing of clioquinol-evolved C. auris strains

To evaluate the ability of Candida auris to develop resistance to clioquinol, and to assess the resulting determinants of resistance, three independent cultures of C. auris were serially passaged 30 times (roughly 150-200 generations) in the presence of increasing concentrations of clioquinol. Whole genome sequencing was performed on two single colony isolates from the endpoint of the resistance experiment for each of the three cultures as well as from populations harvested from selected intermediate points associated with changes in resistance in order to identify the determinant(s) of clioquinol resistance.