Possible bactericidal mechanism of HBc147-183.
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(A) SYTOX Green uptake of P. aeruginosa (circle), K. pneumoniae (cross) E. coli (square), and S. aureus (triangle) by HBc147-183. Measurements of the fluorescence were recorded every minute. (B) Dose-dependent curves of membrane permeabilization of P. aeruginosa by HBc147-183 (black) and HBc153-176 (grey) at 0.5, 1 and 2 µM (dots, dashes and solid line). 2 µM melittin (diamond) was used as a positive control. Samples were measured in triplicates. (C) DNA-binding activity of HBc147-183. HBc147-183 was mixed with pSUPER plasmid DNA at indicated N/P ratio for 30 minutes. The mobility of DNA was determined by gel retardation assay.
创建时间：
2016-02-24
