Images were collected at 4-minute intervals on a DeltaVision system equipped with a with a 60x PlanApo PH3 phase-contrast, oil immersion objective (NA = 1.4). This system has a motorized x-y-z stage,
Optical section series through a protoplasmic astrocyte in rat hippocampal area CA1 intracellulaly injected Lucifer Yellow and imaged with confocal microscopy