Validation of SAGE-type library comparison results and analysis of expression levels of genes encoding proteins involved in infection thread formation by quantitative real-time polymerase chain reaction (qPCR).

Values for qPCR results represent means of three biological replicates. *Contigs were originally classified based on 454 sequencing results. The tendency (nodule-enhanced or root-enhanced expression with R>10 based on the evaluation of the SAGE-type libraries) is considered to be confirmed when the fold change ≥2 (for nodule-enhanced genes) and ≤−2 (for root-enhanced genes) and the difference is significant, p<0.05. Bold indicates p<0.05; bold underline indicates p<0.0019 (Bonferroni correction). †Due to R<10.