Identification of RNAs that are physically and functionally associated with RNase P. Saccharomyces cerevisiae

Numerous RNAs copurify with RNase P and are affected by temperture sensitive mutations in conserved residues with the essential RNA and protein subunits. Specifically, RNase P physically interacts with ribosomal protein mRNAS and the intron-encoded box C/D snoRNAs. Keywords: RNA copurification, temperature sensitive mutants Overall design: ORF and intergenic regions were analyzed in order to determine which RNAs were affected in RNase P strains.