Whole-genome Nanopore sequencing for native mtDNA methylation analysis

We aim to establish and validate a workflow to detect mitochondrial DNA methylation from blood- and iPSC neuron-derived DNA.Therefore blood-derived DNA from five patients with Parkinson's disease and three control subjects was analyzed. As well as DNA from iPSC-derived midbrain neurons from four patients and three controls.To validate the pipeline, fully methylated and unmethylated synthetic DNA was analyzed at different dilution ratios (0%, 50%, 100%). Further, to get a baseline for false-positive methylation calls, two overlapping long-range PCR amplicons of the mtDNA were analyzed as well.