The CRISPR-Cas9 system has become the most promising and versatile tool for genetic manipulation applications. Albeit the technology has been broadly adopted by both academic and pharmaceutic societie
gRNA sites with a 3' GG motif adjacent to the PAM site have been shown to exhibit substantially higher genome-editing efficiency in C. elegans. I scanned 6 model genomes (Saccharomyces cerevisiae, Cae
Supplementary Material 1: Supplementary Tables. Table S1: Quantitative and qualitative comparisons of the GuideScan2 algorithm to existing software for gRNA design and analysis. Table S2: Parameters o