Berberine protects against sepsis-related acute lung injury in rats

Aim: To assess the therapeutic effects of berberine in experimentally induced lung sepsis. Examining its effects on selected cytokines, gene and protein expression and the histopathological findings. Results: Berberine significantly reduced the wet/dry lung ratio, the BALF protein, cell, and neutrophils percentage & the BALF cytokines levels. In addition, pretreatment with berberine decreases the myeloperoxidase and MDA levels and decreases gene expression of NFKB, MCP -1 and the ICAM - 1 by RT - PCR analysis. Thus, suggesting an antioxidant and anti-inflammatory mode of the action. Western blot analysis revealed increased PPAR – Î¥ expression in the berberine pretreated group compared to the CLP group. Histopathological examination revealed improved histopathological parameters, in the berberine pretreated group, compared to the CLP group. Conclusion: Berberine improves the outcome in sepsis via antioxidant and anti-inflammatory effects, modulation of cytokine levels, upregulation ..., , , # Berberine protects against sepsis-related acute lung injury in rats Give a brief summary of dataset contents, contextualized in experimental procedures and results. ## Description of the data and file structure One data file **statistics.docx** with tables and figures.### 研究目的 本研究旨在评估小檗碱（berberine）对实验诱导性肺脓毒症的治疗效果，并考察其对选定细胞因子、基因与蛋白表达的影响，以及组织病理学检测结果。 ### 研究结果 小檗碱可显著降低肺湿干重比值、支气管肺泡灌洗液（bronchoalveolar lavage fluid, BALF）中的蛋白含量、细胞总数及中性粒细胞占比，同时下调灌洗液中细胞因子水平。此外，小檗碱预处理可降低髓过氧化物酶（myeloperoxidase, MPO）与丙二醛（malondialdehyde, MDA）水平；经实时荧光定量聚合酶链反应（reverse transcription-polymerase chain reaction, RT-PCR）分析证实，其可抑制核因子κB（nuclear factor kappa-B, NF-κB）、单核细胞趋化蛋白-1（monocyte chemoattractant protein-1, MCP-1）及细胞间黏附分子-1（intercellular adhesion molecule-1, ICAM-1）的基因表达，提示小檗碱通过抗氧化与抗炎途径发挥作用。蛋白质印迹（Western blot）分析显示，与盲肠结扎穿刺术（cecal ligation and puncture, CLP）模型组相比，小檗碱预处理组的过氧化物酶体增殖物激活受体γ（peroxisome proliferator-activated receptor gamma, PPAR-γ）表达水平显著升高。组织病理学检查结果表明，小檗碱预处理组的组织病理学参数较CLP组得到显著改善。 ### 研究结论 小檗碱可通过抗氧化、抗炎作用，调节细胞因子水平，上调相关通路（原文表述未完整）以改善脓毒症预后。本研究的正式论文题为《小檗碱对大鼠脓毒症相关急性肺损伤的保护作用》。 ### 数据集内容简要概述 请结合实验流程与研究结果，对数据集内容进行简要概括。 ### 数据与文件结构说明 本数据集包含1份包含表格与插图的统计文档（statistics.docx）。