Genome-wide identification of protein phosphatase 2C family members in <i>Glycyrrhiza uralensis</i> Fisch. and their response to abscisic acid and polyethylene glycol stress

The protein phosphatase 2C (PP2C) gene family, which is known to participate in cellular processes, is one of the most conserved plant-specific gene families that regulate signal transduction in eukaryotic organisms. However, the PP2C gene family of <i>Glycyrrhiza uralensis</i> Fisch. has not yet been systematically characterized. In this study, 76 PP2C genes of <i>G. uralensis</i> (<i>GuPP2C</i>s) were identified. Phylogenetic analysis divided these <i>GuPP2C</i>s into 12 subfamilies. The gene structure and protein motifs of the <i>GuPP2C</i>s were then analysed. The analysis of the transcriptomic data of the <i>GuPP2C</i>s revealed that nine genes were highly sensitive to abscisic acid (ABA) stress and might respond to ABA treatment. These nine genes were validated via quantitative real-time polymerase chain reaction. This study provides a foundation for future studies on the functional PP2C genes of <i>G. uralensis</i> and the improvement of the cultivation and quality of <i>G. uralensis</i>.蛋白磷酸酶2C（protein phosphatase 2C, PP2C）基因家族是一类已知参与细胞生理过程的基因家族，亦是真核生物中调控信号转导的最为保守的植物特异性基因家族之一。然而，目前尚未对乌拉尔甘草（Glycyrrhiza uralensis Fisch.）的PP2C基因家族开展系统性的功能鉴定与解析。本研究共鉴定得到76个乌拉尔甘草PP2C基因（命名为GuPP2Cs）。系统发育分析将这些GuPP2C基因划分为12个亚家族。随后对GuPP2C基因的基因结构与蛋白基序进行了分析。对GuPP2C基因的转录组数据分析显示，有9个基因对脱落酸（abscisic acid, ABA）胁迫具有极高敏感性，且可能响应ABA处理。研究通过实时定量聚合酶链式反应对这9个基因进行了验证。本研究为后续乌拉尔甘草PP2C功能基因的研究，以及乌拉尔甘草的栽培与品质改良提供了重要理论基础。

搜集汇总

背景与挑战

背景概述

该数据集总结了甘草中PP2C基因家族的全基因组鉴定结果，共识别出76个基因，分为12个亚家族，并发现其中9个基因对脱落酸胁迫敏感，可能参与胁迫响应。研究为甘草功能基因研究和栽培改良提供了基础。