<b>Figure 5. Intact STING and MAVS signaling is required for Phase I HSV-1 gene expression.</b>
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5A-B. Latently infected neurons were transduced with vectors expressing shSTING or shCTRL during latency. <br>5A. Relative expression of <i>ICP27</i> mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) with LY294002 (20μM). <br>5B. Relative expression of <i>Sting</i> mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) with LY294002 (20μM). <br>5C. Titers of infectious virus at 24 hours post-infection of Wild-type, STING KO, or MAVS KO ARPE-19 cells infected with KOS-SPA or KOS-UL98 at an MOI of 5 PFU/cell. 5D-E. Latently infected neurons were transduced with vectors expressing shMAVS or shCTRL during latency. <br>5D. Relative expression of <i>ICP27</i> mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) with LY294002 (20μM). <br>5E. Relative expression of <i>Mavs</i> mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) with LY294002 (20μM). <br>5F. Relative expression of <i>ICP27</i> mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) following depletion of both STING and MAVS.
