2010/11 VMS Geonomics sampling - data collected from the VMS (Voyage Marine Science) voyage of the Aurora Australis

Purpose of future metagenomic (DNA), metaproteomic (protein) and metatranscriptomic (RNA) analysis:For each sample, two drums (~200L each) of seawater were collected. Samples were taken from CTD sites, and surface samples (2m depth) taken at each of these sites. At most of these CTD sites, a deeper sample was taken according to the location of the DCM at that site. The 200L seawater is pumped through a 20 micron mesh to remove the largest particles, then the biomass is collected on three consecutive filters corresponding to decreasing pore size (3.0 microns, 0.8 microns, 0.1 microns). This is repeated for each sample using the second 200L of seawater to generate duplicates for each sample. The overall aim is to determine the identity of microbes present in the Southern Ocean, and what microbial metabolic processes are in operation. In other words: who is there, and what they are doing. Special emphasis was placed on the SR3 transect.Samples were collected as below. For each sample, a total of six filters were obtained (3x pore sizes, 2x replicates). Each filter is stored in a storage buffer in a 50mL tube, and placed at -80 degrees C for the remainder of the voyage.本数据集用于后续宏基因组学（metagenomic，对应DNA）、宏蛋白质组学（metaproteomic，对应蛋白质）与宏转录组学（metatranscriptomic，对应RNA）分析：针对每个采样点，采集两桶（每桶约200升）海水。采样点位取自温盐深剖面仪（Conductivity Temperature Depth, CTD）站位，且每个站位均采集表层海水样本（水深2米）。在多数上述CTD站位，会根据该站位的深层叶绿素最大值层（Deep Chlorophyll Maximum, DCM）位置采集深层海水样本。将200升海水通过20微米孔径筛网过滤以去除最大粒径颗粒物，随后通过孔径依次递减的三层连续滤膜收集生物量，三层滤膜孔径分别为3.0微米、0.8微米与0.1微米。针对每个采样点，使用第二桶200升海水重复上述操作，以获取该样本的平行重复样。本研究的总体目标为明确南大洋中存在的微生物种类，以及其正在运行的微生物代谢过程，简言之即：探明群落中的微生物组成及其代谢活性。研究重点聚焦于SR3断面。样本采集流程如下：针对每个采样点，共可获取六片滤膜（对应3种孔径与2组平行重复）。将每片滤膜置于装有保存缓冲液的50毫升离心管中，并在航行剩余时段内保存于-80℃环境中。