Supplementary material.docx

Experimental.Plant materials and phytopharmaceutical productChemicals and apparatusPreparation of Standards and samplesInstrumentation and Chromatographic conditionsFiguresOptimisation of chromatographic conditionsS1:Effect of organic solvent type in the mobile phase on separation S2:Effect of initial and final concentration of ACN in the mobile phase on separation S3:Effect of gradient time on separation S4:Effect of column temperature on separation S5:Effect of flow rate on corrected peak area S6:Peak characterization in the crude extracts Indirect-dispersive liquid-liquid microextraction S7: Effect of IDLLME on the baseline, (a) before (b) after IDLLME, Peaks: 1, vitexin 2''-O-rhamnoside, 2, rutin, 3, vitexin, and 4, hyperoside. TablesS1:Optimum chromatographic conditions Determination of analytes in real samples S2:Concentration of analytes in the real samples实验部分 1. 实验材料与植物源药物制剂 2. 化学试剂与仪器设备 3. 标准品与样品制备 4. 仪器配置与色谱条件 5. 附图 5.1 色谱条件优化 图S1：流动相有机溶剂种类对分离效果的影响 图S2：流动相中乙腈（ACN）初始与终浓度对分离效果的影响 图S3：梯度洗脱时间对分离效果的影响 图S4：柱温对分离效果的影响 图S5：流速对校正峰面积的影响 图S6：粗提物中的峰表征 间接分散液液微萃取（Indirect-dispersive liquid-liquid microextraction, IDLLME）： 图S7：IDLLME对基线的影响，(a) 萃取前，(b) 萃取后；峰1：牡荆素2''-O-鼠李糖苷（vitexin 2''-O-rhamnoside），峰2：芦丁（rutin），峰3：牡荆素（vitexin），峰4：金丝桃苷（hyperoside） 6. 附表 表S1：最优色谱条件 实际样品中分析物的测定 表S2：实际样品中分析物的浓度