Figure5_1E_tdTomatoTranslocation_PlusKaps
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Cells were incubated with an ATP-regenerating system, Ran-GTP, NTF2, and an NLS-TD-Tomato cargo in the presence and absence of Kap1- α and Kap1-b.<b> </b>Amount of NLS-TD-Tomato accumulated in the nucleus was measured by quantifying the average fluorescence signal in the nucleus, as defined via Hoechst staining.
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2020-12-07
