Phenotyping and genotyping of recombinant inbred lines and related parents of common bean (Phaseolus vulgaris L.) segregating for seed protein composition

The present dataset includes data acquired in support of a study whose objective was to determine whether a variation in seed protein composition can be used to improve the composition of essential amino acids, particularly the sulphur amino acids, methionine and cysteine, in the seed of common bean (Phaseolus vulgaris L.). The dataset includes a file (Table1_SuppInfo) combining phenotypic data on storage protein profile and amino acid concentrations with genotyping data obtained with the Infinium II BARCBean6K_3 BeadChip (Song et al. 2015 G3 5, 2285; doi:10.1534/g3.115.020594) for a population of recombinant inbred lines derived from a cross between Morden003 and SMARC1N-PN1, along with the following parental and related genotypes: Sanilac, SARC1, SMARC1-PN1, Great Northern US 1400, three Phaseolus coccineus accessions deficient in phaseolin and the wild P. vulgaris accession G12882. The following files are included: Dataset1_SuppInfo. SDS-PAGE of protein profiles of parents and recombinant inbred lines. M: Morden003; S: SMARC1N-PN1. Pop1 is indicated by the prefix F08-18-01 followed by a number; Pop2 by the prefix F08-18-02; and Pop3 by the prefix W09-01-01. The protein genotype is indicated by a color-coded arrow: blue, Morden003 genotype at both phaseolin and lectin loci (MM); green, Morden003 genotype at the phaseolin locus and SMARC1N-PN1 genotype at the lectin locus (MS); red, SMARC1N-PN1 genotype at the phaseolin locus and Morden003 genotype at the lectin locus (SM); and black, SMARC1N-PN1 genotype at both phaseolin and lectin loci (SS). Table1_SuppInfo. Genotypic and phenotypic information of parents, RILs and related genotypes. High-density SNP genotyping information is provided, along with the genotype at the phaseolin and lectin loci, and the concentration of cysteine, methionine, cysteine + methionine and S-methylcysteine. Average; n = 3. Table2_SuppInfo. Concentration of cysteine, methionine, cysteine +methionine and S-methylcysteine for a subgroup of RILs that was determined at the SPARC BioCentre of SickKids Hospital, Toronto, ON. Figure1_SuppInfo. Graphical representation of the genetic map of the Morden003 × SMARC1N-PN1 recombinant inbred population. Table3_SuppInfo. Information on SNP markers used to track phaseolin or lectin deficiency. Top panel provides genotyping results. Lower panel provides allele information. Table4_SuppInfo. Primer information of SNP markers used to track phaseolin or lectin deficiency. Dataset2_SuppInfo. Genotyping data obtained during the validation of marker ss715646356. RFU: relative fluorescence units. Dataset3_SuppInfo. Genotyping data obtained during the validation of marker ss715647037. RFU: relative fluorescence units. Dataset4_SuppInfo. Genotyping data obtained during the validation of marker ss715648961. RFU: relative fluorescence units. Dataset5_SuppInfo. Melt curves of SNP primers used with the (Tm)-shift SNP genotyping method. Dataset6_SuppInfo. Amino acid and protein profiles of each field plot for 7 SS lines at Morden (MF) and London (LF) (2014 and 2015).本数据集包含为支撑一项研究而获取的实验数据，该研究旨在探究种子蛋白质组成的变异是否可用于改善普通菜豆（*Phaseolus vulgaris* L.）种子中必需氨基酸，尤其是含硫氨基酸——甲硫氨酸与半胱氨酸的组成。数据集包含文件Table1_SuppInfo，该文件整合了贮藏蛋白质表型谱、氨基酸浓度数据，以及使用Infinium II BARCBean6K_3基因芯片（Song等，2015，*G3* 5, 2285；doi:10.1534/g3.115.020594）获得的基因型数据，对应群体为Morden003与SMARC1N-PN1杂交衍生的重组自交系（recombinant inbred line, RIL）群体，同时包含以下亲本及相关基因型：Sanilac、SARC1、SMARC1-PN1、Great Northern US 1400、3份缺失伴大豆球蛋白的红花菜豆（*Phaseolus coccineus*）种质，以及野生普通菜豆种质G12882。 本次数据集包含以下文件： 1. Dataset1_SuppInfo：亲本与重组自交系的蛋白质谱十二烷基硫酸钠聚丙烯酰胺凝胶电泳（Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, SDS-PAGE）结果。其中M代表Morden003，S代表SMARC1N-PN1。群体1以前缀F08-18-01加编号标识，群体2以F08-18-02为前缀，群体3以W09-01-01为前缀。蛋白质基因型由颜色编码箭头标注：蓝色代表在伴大豆球蛋白与凝集素位点均为Morden003基因型（MM）；绿色代表伴大豆球蛋白位点为Morden003基因型、凝集素位点为SMARC1N-PN1基因型（MS）；红色代表伴大豆球蛋白位点为SMARC1N-PN1基因型、凝集素位点为Morden003基因型（SM）；黑色代表在伴大豆球蛋白与凝集素位点均为SMARC1N-PN1基因型（SS）。 2. Table1_SuppInfo：亲本、重组自交系及相关基因型的基因型与表型信息。包含高密度单核苷酸多态性（Single Nucleotide Polymorphism, SNP）基因型数据，以及伴大豆球蛋白、凝集素位点的基因型，还有半胱氨酸、甲硫氨酸、半胱氨酸+甲硫氨酸与S-甲基半胱氨酸的浓度数据。平均值；n=3。 3. Table2_SuppInfo：部分重组自交系的半胱氨酸、甲硫氨酸、半胱氨酸+甲硫氨酸及S-甲基半胱氨酸浓度数据，该批次数据由加拿大多伦多病童医院SPARC生物中心测定。 4. Figure1_SuppInfo：Morden003 × SMARC1N-PN1重组自交群体遗传图谱的图形化展示。 5. Table3_SuppInfo：用于追踪伴大豆球蛋白或凝集素缺失的SNP标记信息。上半部分为基因型分型结果，下半部分为等位基因信息。 6. Table4_SuppInfo：用于追踪伴大豆球蛋白或凝集素缺失的SNP标记的引物信息。 7. Dataset2_SuppInfo：标记ss715646356验证过程中获得的基因型数据。RFU：相对荧光单位（relative fluorescence units）。 8. Dataset3_SuppInfo：标记ss715647037验证过程中获得的基因型数据。RFU：相对荧光单位。 9. Dataset4_SuppInfo：标记ss715648961验证过程中获得的基因型数据。RFU：相对荧光单位。 10. Dataset5_SuppInfo：采用（Tm）位移SNP基因分型法的SNP引物解链曲线数据。 11. Dataset6_SuppInfo：2014年与2015年，在莫登（MF）与伦敦（LF）两地的7个SS系各田间小区的氨基酸与蛋白质组成数据。