Additional file 1 of What evidence exists for the impact of climate change on the physiology and behaviour of important aquaculture marine crustacean species in Asia? A systematic map protocol
Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performe