(A) Double knock-out of pax6 and tyr was achieved by TALEN mRNA injection to GV oocytes. A double knock-out tadpole is shown. (B) Detection of pax6 and tyr mutants by RFLP analysis. Wild type embryos
Protein lysates were collected from WT or Xrn1 ko A549 cells and HEKT293T ishXrn1 cells treated with doxycycline for 3-4 days or left untreated. They were then probed with antibodies against Xrn1 or b
