36B4 annealing temperature varied according to target gene being analyzed. IL-6, interleukin-6; IL-10, interleukin-10; MCP-1, monocyte chemoattractant protein-1; TNFα, tumor necrosis factor alpha; and
RT-qPCR average Cq for 20 DSLD horse skin samples and 18 control horse skin samples. The Cq values were average of total 20 DSLD horse skin samples and 18 normal horse skin samples. Each sample was ru
