Data from: A transgenic quail model that enables dynamic imaging of amniote embryogenesis

Embryogenesis is the coordinated assembly of tissues during morphogenesis through changes in individual cell behaviors and collective cell movements. Dynamic imaging, combined with quantitative analysis, is ideal for investigating fundamental questions in developmental biology involving cellular differentiation, growth control and morphogenesis. However, a reliable amniote model system that is amenable to the rigors of extended, high-resolution imaging and cell tracking has been lacking. To address this shortcoming, we produced a novel transgenic quail that ubiquitously expresses nuclear localized monomer cherry fluorescent protein (chFP). We characterize the expression pattern of chFP and provide concrete examples of how Tg(PGK1:H2B-chFP) quail can be used to dynamically image and analyze key morphogenetic events during embryonic stages X to 11.胚胎发生（Embryogenesis）是指在形态发生（Morphogenesis）过程中，通过单个细胞行为改变与集体细胞运动，实现组织协同组装的生物学过程。动态成像结合定量分析，是探究发育生物学中涉及细胞分化、生长调控与形态发生等核心科学问题的理想研究手段。然而，目前仍缺乏能够适配长期、高分辨率成像与细胞追踪严苛实验要求的可靠羊膜动物（Amniote）模型系统。为弥补这一研究短板，我们构建了一种新型转基因鹌鹑，其可遍在表达定位于细胞核的单体樱桃荧光蛋白（Monomer Cherry Fluorescent Protein，chFP）。我们对chFP的表达模式进行了系统表征，并通过具体实例展示了Tg(PGK1:H2B-chFP)转基因鹌鹑可用于动态成像与分析胚胎发育阶段X至11期的关键形态发生事件。