Supporting data for "NEXT-scASV: A Nextflow Pipeline for Allele-Specific Variants Calling from single cell RNA-seq data"

NEXT-scASV (Nextflow pipeline for Allele-Specific Variant calling), a scalable Nextflow pipeline for calling allele-specific variants (ASVs) from 5' single-cell RNA sequencing data. NEXT-scASV integrates established tools for alignment (HISAT2), reads deduplication (umi_tools), variant calling (bcftools mpileup), reference bias correction (WASP), and advanced statistical modeling (MIXALIME). The pipeline consists of five main subflows: (i) Data Splitting, (ii) Alignment and Filtering, (iii) Variant Calling, (iv) Allelic Read Counting, and (v) ASV Calling.<br>Dataset contains processed test data from different processing steps: initinal variant calling, allele counting, AS variants calling