SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in May 2016

Flow cytometry data was collected in May 2016, in waters off South Australia. The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.本数据集的流式细胞术（Flow Cytometry）数据采集于2016年5月，采集区域为澳大利亚南部近岸海域。本研究的核心目标为建立澳大利亚南部大陆架海域生态系统的背景知识库，阐明上升流/高盐冲溢事件对微生物群落的影响，最终构建该区域的生物地球化学模型。采样工作依托恩格林号科考船（RV Ngerin）开展，属于南澳大利亚综合海洋系统（SAIMOS）的研究内容之一。每次科考航次中，研究人员均对叶绿素荧光最大值层（Deep Chlorophyll Maximum, DCM）的物理、化学与生物学特性展开调查。本数据集针对微微型浮游植物、细菌与病毒采集了流式细胞术数据。本研究共设置6个主要采样站位：其中5个站位位于100米等深线海域，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）以及SAM5CB/B5（35.008°S，135.198°E）；剩余1个站位为位于袋鼠岛西南侧的外海站位B1（36.188°S，136.178°E）。需说明的是，结合站位间距（14~25海里）、陆架中层海流平均流速分量（0.01 m·s⁻¹）以及科考船平均航速（9节），可推断各站位采集的水体团均不相同。研究期间还偶尔于袋鼠岛国家参考站位（National Reference Station, NRS，35.832°S，136.447°E）以及发生高盐冲溢事件的南澳大利亚斯宾塞湾口锚泊站（SAM8SG，35.25°S，136.690°E）采集了补充样本。