Differentially expressed (DE) genes analysis in synovial fluid mesenchymal stem cells (SF-MSCs), SF-MSC derived iPSCs and iPSC derived MSCs (iPSC-MSCs)

We report the application of next-generation sequencing (NGS) to analysis the gene expression profile among three different cells:SF-MSCs, iPSCs and iPSC-MSCs. Our results shown that iPSC-MSCs were more similar to SF-MSCs than iPSCs. Human SF-MSCs were reprogrammed through the non-viral, integration-free episomal approach into induced pluripotent stem cells (iPSCs), which were then subsequently differentiated into MSCs to establish iPSC-MSC lines.