Data from: Karyotype analysis of four jewel-beetle species (Coleoptera, Buprestidae) detected by standard staining, C-banding, AgNOR-banding and CMA3/DAPI staining

The male karyotypes of Acmaeodera pilosellae persica Mannerheim, 1837 with 2n=20 (18+neoXY), Sphenoptera scovitzii Faldermann, 1835 (2n=38–46), Dicerca aenea validiuscula Semenov, 1895 – 2n=20 (18+Xyp) and Sphaerobothris aghababiani Volkovitsh et Kalashian, 1998 – 2n=16 (14+Xyp) were studied using conventional staining and different chromosome banding techniques: C-banding, AgNOR-banding, as well as fluorochrome Chromomycin A3 (CMA3) and DAPI. It is shown that C-positive segments are weakly visible in all four species which indicates a small amount of constitutive heterochromatin (CH). There were no signals after DAPI staining and some positive signals were discovered using CMA3 staining demonstrating absence of AT-rich DNA and presence of GC-rich clusters of CH. Nucleolus organizing regions (NORs) were revealed using Ag-NOR technique; argentophilic material mostly coincides with positive signals obtained using CMA3 staining.本研究采用常规染色法、C带染色、AgNOR染色以及荧光染料色霉素A3（Chromomycin A3, CMA3）与DAPI染色等多种染色体显带技术，对4种昆虫的雄性核型展开分析，涉及物种及核型信息如下：Acmaeodera pilosellae persica Mannerheim, 1837（2n=20，18+neoXY）、Sphenoptera scovitzii Faldermann, 1835（2n=38~46）、Dicerca aenea validiuscula Semenov, 1895（2n=20，18+Xyp）以及Sphaerobothris aghababiani Volkovitsh et Kalashian, 1998（2n=16，14+Xyp）。研究结果表明，4个物种的C带阳性区段均表现较弱，提示其组成型异染色质（constitutive heterochromatin, CH）含量偏低。经DAPI染色未检测到阳性信号，而CMA3染色则观测到部分阳性信号，这证明该类群基因组中不存在AT富集DNA区域，却存在GC富集的组成型异染色质簇。通过Ag-NOR技术可检出核仁组织区（nucleolus organizing regions, NORs），其嗜银物质大多与CMA3染色得到的阳性信号共定位。