Persistence of sub-clinical <em>Batrachochytrium dendrobatidis</em> infection in stream anurans in South Asia

Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and contributes significantly to amphibian declines globally. It has affected more than 500 amphibian species on five continents. In South Asia, the Western Ghats is an amphibian biodiversity hotspot; however, the population status of anurans and their burden of chytridiomycosis are not well understood. Our aim was to understand pathogen prevalence, load, and its persistence in a stream anuran community in the Western Ghats. We carried out a capture-mark-recapture (CMR) programme in five ephemeral streams of the Tillari Conservation Reserve (TCR), Western Ghats, India, from 2018 to 2020 during the early (wet) and late (dry) recovery periods. We used quantitative Polymerase Chain Reaction (qPCR) to assign disease states - no (N), low (L), and High (H) levels to every anuran, based on the Bd loads detected. We constructed population models using CMR data from skittering frogs, with Program MARK, and estimated transition pro..., , # Persistence of sub-clinical *Batrachochytrium dendrobatidis* infection in stream anurans in South Asia

Dataset DOI: [10.5061/dryad.1zcrjdg5v](10.5061/dryad.1zcrjdg5v)

## Description of the data and file structure

File: Field_Data_2018_to_2020.xlsx

It contains data used for capture mark recapture of frogs from 5 stream segments in Tillari Conservation Reserve, Maharashtra, India, from 2018-2020. The data represent frogs that were captured and marked, and recaptured, that were > 29 mm in snout vent length. Descriptions of the data are given below.

Session : Odd numbers are Wet session, and even numbers are Dry session
Stream : HB_Hevale Bridge, KA_Kharade, HV_Hevale village, GW_Ghatiwade, RW_Raniwade
Visit : Total 5 visits each session
R_N : Recapture( R ), and New Capture ( N )
Swabb : Yes taken ( Y ), and Not taken ( N )
Wt : Weight in grams
SVL : Snout to Vent length in mm
T : Vertical transect quadrant number
B : Horizontal transect quadrant number
DNA Serial No : DNA Seria...,

壶菌病（Chytridiomycosis）由蛙壶菌（Batrachochytrium dendrobatidis, Bd）引发，是全球两栖动物种群衰退的关键驱动因素之一，已影响五大洲逾500种两栖动物。南亚的西高止山脉（Western Ghats）是两栖动物生物多样性热点区域，但目前该区域无尾两栖类（anurans）的种群现状及其壶菌病感染负担仍不甚明晰。本研究旨在明确西高止山脉溪流无尾两栖类群落中病原体的感染率、载量及其持续存在特征。

2018年至2020年，我们于印度西高止山脉蒂拉里保护保护区（Tillari Conservation Reserve, TCR）的5条季节性溪流中，分别在湿季早期与干季晚期开展标记-重捕（capture-mark-recapture, CMR）实验，其中湿季对应湿润恢复期、干季对应干旱恢复期。基于检测得到的Bd载量，通过实时荧光定量聚合酶链反应（quantitative Polymerase Chain Reaction, qPCR）为每只无尾两栖类划分感染等级：无感染（N）、低载量感染（L）与高载量感染（H）。我们使用Program MARK软件，基于滑栖蛙类（skittering frogs）的标记重捕数据构建种群模型，并估算种群转换率……

# 南亚溪流无尾两栖类亚临床蛙壶菌感染的持续存在
数据集DOI：10.5061/dryad.1zcrjdg5v

## 数据与文件结构说明
文件：Field_Data_2018_to_2020.xlsx
该文件包含2018年至2020年于印度马哈拉施特拉邦蒂拉里保护保护区5条溪流河段开展的两栖类标记重捕实验数据，涵盖吻肛长大于29 mm的捕获、标记及重捕个体。下文对数据字段进行详细说明：
Session（采样时段）：奇数编号对应湿季采样时段，偶数编号对应干季采样时段
Stream（溪流编号与名称）：HB_Hevale Bridge（赫瓦莱桥）、KA_Kharade（卡拉德）、HV_Hevale village（赫瓦莱村）、GW_Ghatiwade（加蒂瓦德）、RW_Raniwade（拉尼瓦德）
Visit（采样次数）：每个采样时段共开展5次采样
R_N（捕获类型）：R代表重捕个体，N代表新捕获个体
Swabb（是否取样）：Y为已完成皮肤取样，N为未取样
Wt（体重）：单位为克（g）
SVL（吻肛长）：单位为毫米（mm）
T（垂直样带象限编号）
B（水平样带象限编号）
DNA Serial No（DNA序列号）：……