CIL:7352, notopthalmus viridescens, erythrocyte. In Cell Image Library

Newt (Notopthalmus viridescens erythrocytes were isolated in 0.1M KCl, spread on the surface of Na-citrate, picked up on formvar-carbon films, fixed with glutaraldehyde and negatively stained with 2% uranyl acetate. Images were recorded at 40KX and accelerating voltage of 1MeV at the University of Wisconsin HVEM facility.This image was taken with a specimen tilt of 45 degrees. A grouped image of the same field at a 55 degree tilt provides an oblique stereo view of the tangle of chromatin fibers in the dispersed nucleus.本数据集以美洲红蝾螈（Notopthalmus viridescens）的红细胞为实验材料：将红细胞在0.1M氯化钾（KCl）溶液中分离后，铺展于柠檬酸钠（Na-citrate）表面，再用福尔瓦-碳复合支撑膜捞取样品，经戊二醛固定后，以2%醋酸铀酰溶液进行负染色处理。成像工作于威斯康星大学高压电子显微镜（HVEM）平台开展，图像采集参数为40,000倍（40KX）放大倍率、1MeV加速电压，本次成像的样品倾斜角度为45度。同一场景下以55度倾斜角度拍摄的分组图像，可呈现分散细胞核内染色质纤维缠结结构的斜视角立体视图。