HTS DB of Nanomaterials on HepaRGs

The large amount of existing nanomaterials demands for rapid and reliable ways to test their potential toxicological effect on human health, preferably by means of relevant in vitro tests in order to reduce testing on animals. Combining high throughput workflows with automated high content imaging techniques allows to derive much more information from cell-based assays than with the typical readouts (i.e. one measurement per well) with optical plate-readers. We present here a dataset including up to 14 different read outs including viable cell count, cell membrane permeability, apoptotic cell death, mitochondrial membrane potential and steatosis of the human hepatoma HepaRG cell line treated with a large set of nanomaterials, coatings and supernatants at different concentrations. The database, given its size, can be utilized for the development of in silico hazard assessment and prediction tools or can be combined with toxicity effect on other in vitro test systems.

搜集汇总

背景与挑战

背景概述

该数据集是高内涵成像数据库，记录了纳米材料在人类肝癌HepaRG细胞系上的毒性效应，包含多达14种不同的生物学读数（如细胞活力、膜损伤、凋亡等）。该数据库规模较大，可用于构建计算机毒性预测模型或与其他体外测试数据结合分析。