Immunoreactive peptide maps of SARS-CoV-2

Serodiagnosis of SARS-CoV-2 infection is impeded by immunological cross-reactivity among the human coronaviruses (HCoVs) SARS-CoV-2, SARS-CoV-1, MERS-CoV, OC43, 229E, HKU1, and NL63. To study humoral immune responses specific to SARS-CoV-2, it is imperative to identify peptides that may enable discrimination between exposure to SARS-CoV-2 and other HCoVs. We used a high-density peptide microarray and plasma samples collected at two time points from 50 subjects with SARS-CoV-2 infection confirmed by qPCR, samples collected in 2004-2005 from 11 subjects with IgG antibodies to SARS-CoV-1, 11 subjects with IgG antibodies to other seasonal human coronaviruses (HCoV), and 10 healthy human subjects. The peptide microarrays consist of ~172000 12-mer peptide probes spanning whole viral genomes. This dataset consists of reactivity values for individual peptides from all 132 plasma samples for both IgG and IgM. Also made available is the correspondence key that provides a reference for all viral g...严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）感染的血清学诊断，会受到人冠状病毒（Human Coronaviruses, HCoVs）家族各成员间免疫交叉反应的干扰。该家族包含SARS-CoV-2、SARS-CoV-1、中东呼吸综合征冠状病毒（MERS-CoV）、OC43、229E、HKU1以及NL63。为研究针对SARS-CoV-2的特异性体液免疫应答，亟需筛选出可区分SARS-CoV-2暴露与其他HCoV暴露的肽段。本研究采用高密度肽微阵列技术，结合多组血浆样本开展分析：其中50份样本来自经实时荧光定量PCR（quantitative real-time polymerase chain reaction, qPCR）确认感染SARS-CoV-2的受试者，采集于两个时间节点；另有11份样本来自SARS-CoV-1 IgG抗体阳性受试者、11份来自其他季节性人冠状病毒（HCoV）IgG抗体阳性受试者，以及10份健康人血浆样本，上述三组样本均采集于2004-2005年。本次使用的肽微阵列包含约172000条12聚体肽探针，覆盖所有对应病毒的全基因组序列。本数据集包含全部132份血浆样本针对各肽段的IgG与IgM反应活性数值。同步公开的还有用于为所有病毒基因组（原文内容未完整）提供参考的对应索引表。