DOSS Treated Mouse Plasma Adipokine and Cytokine Levels

Pregnant dams were treated at day E11.5 with either 62.5 ug/mL DOSS in drinking water or 0.5% carboxy methyl cellulose (CMC) in drinking water through weaning of pups. Pups were then given normal drinking water until time of sacrifice. Plasma was collected from sacrifice animals and analyzed for adipokine and cytokine levels including Adiponectin, Leptin, Insulin, IL-6 and TNF-alpha. Treated animals exhibit increased IL-6 and TNF-alpha levels in conjunction with decreased Adiponectin levels consistent with a metabolic disruption phenotype and glucose intolerance which was observed in these animals. No changes were observed in plasma leptin and insulin levels, suggesting glucose intolerance is not due to altered insulin levels.本实验中，妊娠母鼠于胚胎发育第E11.5天开始，通过饮水分别给予62.5 μg/mL的DOSS或0.5%羧甲基纤维素（carboxy methyl cellulose, CMC）溶液，持续给药至仔鼠断乳。随后仔鼠饮用常规饮水直至处死取材。采集处死动物的血浆，检测其中的脂肪因子与细胞因子水平，检测指标包括脂联素（Adiponectin）、瘦素（Leptin）、胰岛素（Insulin）、IL-6及TNF-α。给药组动物的血浆IL-6与TNF-α水平升高，同时脂联素水平降低，该变化与本实验中观察到的代谢紊乱表型及糖耐量异常相符。血浆瘦素与胰岛素水平未发生显著变化，提示本次观察到的糖耐量异常并非由胰岛素水平改变所导致。