Raw diffraction images of polyhedra in-vivo crystals
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Diffraction images of wild type cypovirus polyhedra in-vivo crystals (WTPhC) and the mutant (Δ3-PhC) related to PDB codes 5GQM and 5GQN, respectively. Small-wedge (5°/crystal) datasets were collected from loop-harvested microcrystals using EIGER X 9M detector at a wavelength of 1 Å on BL32XU, SPring-8. The datasets for 5GQN were collected automatically using ZOO system. The crystals belonged to space group I23 with unit cell parameter a~103 Å. 14 and 41 datasets were merged at 1.68 and 1.55 Å resolution in the published result (Abe et al. ACS Nano 2017; PDB codes: 5GQM & 5GQN, respectively) using KAMO; see https://github.com/keitaroyam/yamtbx/wiki/Processing-Polyhedra-data-(5GQM-&-5GQN) NOTE flatfield correction was not applied to the images and you need to apply it using the correction table saved in master.h5 files. master.h5 files were modified; see https://github.com/keitaroyam/yamtbx/blob/master/doc/eiger-en.md Most frames have ice (rings).



