Resistance in cowpea cultivars to Bruchidius atrolineatus (PIC) (Coleoptera: Chrysomelidae: Bruchinae)

ABSTRACT: This study aimed to assess the resistance in cowpea cultivars to Bruchidius atrolineatus (Pic) attack. The experiment was conducted at the Faculdade de Ciências Agrárias e Veterinárias of Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Jaboticabal (SP). The following cultivars were used: BR17 Gurgueia, BR3 Tracuateua, BRS Novaera, Sempre Verde, BRS Milênio, and BRS Urubuquara. The non-preference test without choice evaluated the number of viable eggs, unviable and total posed by insects in grains, after 25 days of confinement. In addition, it was observed, in a daily basis, the total dry matter consumed and dry matter consumed per insect; male and female mass; male, female and total egg-adult cycle length; male and female longevity; sex ratio; number of emerged insects and percentage of emerged insects. The non-preference test with free choice evaluated the number of viable, unviable and total eggs and adult attractiveness after 24 hours and seven days of release. Sempre Verde cultivar was the least preferred for oviposition in the test without choice and showed lower number and percentage of emerged insects. BRS Milênio had the lowest total dry mass consumed and dry mass consumed per insect.摘要：本研究旨在评估豇豆品种对线纹豆象（Bruchidius atrolineatus (Pic)）的抗虫性。试验依托圣保罗州立大学"胡利奥·德·梅斯基塔·菲略"（Universidade Estadual Paulista "Júlio de Mesquita Filho", UNESP）农业与兽医学院，于巴西圣保罗州雅博蒂卡瓦尔市开展。供试豇豆品种共6个，分别为BR17古尔盖亚（BR17 Gurgueia）、BR3特拉夸特瓦（BR3 Tracuateua）、BRS Novaera、Sempre Verde、BRS千禧（BRS Milênio）及BRS乌鲁布夸拉（BRS Urubuquara）。 非偏好性无选择试验：将昆虫密闭饲养25天后，统计谷粒上的存活卵数、非存活卵数及总产卵量。同时每日观测如下指标：总干物质消耗量、单头昆虫干物质消耗量、雌雄成虫体重、卵至成虫的发育历期（分雄性、雌性及总周期）、雌雄成虫寿命、性比、羽化昆虫总数及羽化率。 非偏好性自由选择试验：在昆虫释放24小时及7天后，分别统计存活卵数、非存活卵数、总产卵量，并评估成虫趋性。 试验结果显示：Sempre Verde品种在无选择试验中最不易被成虫选择产卵，且羽化昆虫数量与羽化率均处于较低水平；BRS千禧的总干物质消耗量及单虫干物质消耗量均为最低。