Zebrafish embryo developmental abnormalities and mortality in response to DWH mousse concentrations.

This study examined the relationship between DWH mousse amounts (0.0, 25.0, 50.0, 100.0 and 200.0 mg /spread on 16 sq cm2 of surface area) and direct contact toxicity as determined by developmental abnormalities at 96 hours of exposure and cumulative mortality tallied at 192 of exposure. The mousse was spread on the same amount of surface area no matter the total amount applied. The thickness of the mousse varied with the amount applied. The results suggest that increases in amount of mousse on surfaces was not represented with stoichiometric increases in toxicity to zebrafish embryos in direct contact with the mousse as would be observed with substrate breeding fishes. Contact surface was determined by chorionated embryo size.本研究考察了DWH泡沫（DWH mousse）的施用量（0.0、25.0、50.0、100.0及200.0 mg，涂布于16平方厘米的表面区域）与直接接触毒性之间的关联，该毒性通过暴露96小时后出现的发育异常，以及暴露192小时时统计的累积死亡率进行判定。无论总施用量多少，泡沫均涂布于相同面积的表面，其厚度随施用量的增加而变化。研究结果显示，与基质产卵鱼类中的观测结果不同，与该泡沫直接接触的斑马鱼胚胎，其毒性并未随表面泡沫施用量的增加而呈现化学计量比的增长。接触面积由带卵壳的胚胎尺寸决定。