3 samples from Strait of Georgia uncultured phage metagenome. marine metagenome
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GOB1A - isolated from nearshore estuary, photic zone, mixed layer; sample description: sampled off Jericho Pier, Vancouver, British Columbia. PCR amplification of DNA with virus specific primers after 0.45 micron filtration; 30kD tangential flow-filtration; ultracentrifugation pelleting and phenol/chloroform DNA extraction GOB2A - isolated from nearshore estuary, photic zone, mixed layer; sample description: sampled off Point Atkinson. PCR amplification of DNA with virus specific primers after 0.45 micron filtration; 30kD tangential flow-filtration; ultracentrifugation pelleting and phenol/chloroform DNA extraction GOB3A - isolated from coastal sea, photic zone, mixed layer; sample description: sampled during whale watching tour of coast of Vancouver Island. PCR amplification of DNA with virus specific primers after 0.45 micron filtration; 30kD tangential flow-filtration; ultracentrifugation pelleting and phenol/chloroform DNA extraction



