Hypoxia disrupts metabolism in coral and sea anemone larvae
收藏资源简介：
Anthropogenic pollution is driving an increase in the frequency and severity of seawater hypoxic events in coastal marine ecosystems. Although hypoxia decreases physiological performance in coral and sea anemone (phylum Cnidaria) larvae, the underlying cellular mechanisms remain unexplored. Here, larvae of the reef-building corals Galaxea fascicularis and Porites astreoides and the estuarine sea anemone Nematostella vectensis were exposed to normoxia or a simulated hypoxic event (6â h at <2â mg dissolved O2 lâ1), and their metabolomic response was quantified at the end of the exposure period using targeted liquid chromatography-mass spectrometry. Baseline metabolite profiles (81 amino acids, acylcarnitines, organic acids and nucleotides) were broadly divergent between the three species, with the corals displaying a reliance on nitrogen cycling through amino acid metabolism, whereas N. vectensis relied on nucleotide metabolism. By contrast, s..., Groups of larvae were exposed to normoxic or hypoxic conditions for 6 h, flash-frozen, and then processed for LC-MS metabolomics at the University of Pennsylvania Metabolomics Core. Further details can be found in the associated Open Access publication at: https://doi.org/10.1242/jeb.250372., , # Data from: Hypoxia disrupts metabolism in coral and sea anemone larvae This dataset contains targeted LC-MS metabolomics data for coral and sea anemone larvae exposed to normoxic and hypoxic conditions. Further information can be found in the associated Open Access publication at: [https://doi.org/10.1242/jeb.250372](https://doi.org/10.1242/jeb.250372). ## Data and file structure ### File: Metabolomics_data.csv This file contains sample metadata and abundance data for all metabolites quantified at the University of Pennsylvania Metabolomics Core. These files have headers, which include: * Species = the species from which metabolomics data originate * Treatment = experimental treatment (normoxia or hypoxia) * Cohort = the spawning cohort from which larvae originate (not relevant for this dataset) * Group = replicate group within the experimental treatment (1-3) * Life_stage = always \"larva\" * Hours_post_treatment = time point at which larvae were collected for metabolomics; here,...,
人为污染正推动沿海海洋生态系统中海水低氧事件的发生频率与严重程度持续升高。尽管低氧会降低珊瑚与海葵（刺胞动物门（Cnidaria））幼体的生理机能，但其背后的核心细胞机制仍未得到探明。本研究将造礁珊瑚丛生盔形珊瑚（Galaxea fascicularis）、星状微孔珊瑚（Porites astreoides）以及河口海葵艾氏美海葵（Nematostella vectensis）的幼体，分别暴露于常氧环境或模拟低氧事件（溶解氧浓度<2 mg·L⁻¹，暴露6小时）中，并在暴露结束后通过靶向液相色谱-质谱联用（liquid chromatography-mass spectrometry, LC-MS）技术定量分析其代谢组学响应。三种生物的基线代谢物谱（涵盖81种氨基酸、酰基肉碱、有机酸与核苷酸）整体存在显著分化：造礁珊瑚依赖通过氨基酸代谢完成的氮循环，而艾氏美海葵则以核苷酸代谢为主要代谢通路。与此相对，将幼体置于常氧或低氧环境中暴露6小时后，快速冷冻样本，并于宾夕法尼亚大学代谢组学核心实验室开展液相色谱-质谱代谢组学检测。详细实验细节可查阅相关开放获取文献：https://doi.org/10.1242/jeb.250372。 # 数据集来源：低氧干扰珊瑚与海葵幼体的代谢过程 本数据集包含暴露于常氧与低氧环境中的珊瑚及海葵幼体的靶向液相色谱-质谱代谢组学数据。更多相关信息可查阅对应开放获取文献：https://doi.org/10.1242/jeb.250372。 ## 数据与文件结构 ### 文件：Metabolomics_data.csv 该文件包含宾夕法尼亚大学代谢组学核心实验室定量得到的所有代谢物的样本元数据与丰度数据。 该文件带有表头，具体字段包括： * Species：代谢组学数据来源物种 * Treatment：实验处理条件（常氧或低氧） * Cohort：幼体来源的产卵批次（本数据集无需关注该字段） * Group：实验处理内的重复组（编号1-3） * Life_stage：生物个体的生命阶段，固定为"幼体" * Hours_post_treatment：幼体采集用于代谢组学分析的处理后时长；本研究中该参数为……
