Vitamin B5 rewires Th17 cell metabolism via impeding PKM2 nuclear translocation

We validated the interaction between PKM2 and CoA by IP/WB, the immunoblotting showed that CoA strongly interacted with endogenous PKM2. We measured the phosphorylation and nuclear tanslocation of PKM2 with VB5 or CoA treatment.我们通过免疫共沉淀（Immunoprecipitation, IP）与蛋白质印迹（Western Blot, WB）实验，验证了丙酮酸激酶M2（Pyruvate Kinase M2, PKM2）与辅酶A（Coenzyme A, CoA）之间的相互作用。免疫印迹结果显示，辅酶A可与内源性丙酮酸激酶M2发生较强的相互作用。我们检测了经维生素B5（VB5）或辅酶A处理后，丙酮酸激酶M2的磷酸化水平与核转位情况。