Polymer-based surface plasmon resonance biochip: construction and experimental aspects

Abstract Introduction: Surface plasmon resonance biosensors are high sensitive analytical instruments that normally employ glass materials at the optical substrate layer. However, the use of polymer-based substrates is increasing in the last years due to favorable features, like: disposability, ease to construction and low-cost design. Review Recently, a polymer-based SPR biochip was proposed by using monochromatic and polychromatic input sources. Its construction and experimental considerations are detailed here. Experimental considerations and results, aspects from performance characteristics (resonance parameters, sensitivity and full width at half maximum – FWHM – calculations) are presented for hydrophilic and hydrophobic solutions. It is included also a brief description of the state of the art of polymer-based SPR biosensors.摘要与引言：表面等离子体共振生物传感器（Surface Plasmon Resonance Biosensors）是一类高灵敏度分析仪器，其光学基底通常以玻璃为材料。然而近年来，聚合物基底凭借可弃置性、制备简便、设计成本低廉等优良特性，应用愈发广泛。 综述：近期，一项研究提出了采用单色与多色输入光源的聚合物基SPR生物芯片，本文详细阐述了该芯片的制备方案与实验设计要点。针对亲水与疏水溶液，本文给出了实验设计要点与实测结果，涵盖共振参数、灵敏度以及半高全宽（Full Width at Half Maximum, FWHM）计算等性能特征相关内容。此外，本文还简要介绍了聚合物基SPR生物传感器的研究现状。