The relationship between microbial community succession, decay, and anatomical character loss in non-biomineralized animals

A  fundamental assumption of hypothesis-driven decay experiments is that, during decay, the loss of anatomy follows a sequence broadly controlled by the intrinsic compositional properties of tissues. Recent work investigating the succession of postmortem endogenous microbial communities (thanatomicrobiome) challenges this assumption. These studies suggest the internal thanatomicrobiome exhibits a predictable succession in response to physical and chemical environmental changes that occur within a carcass. Therefore, it is possible that reproducible sequences of character loss during decay are controlled by thanatomicrobiome succession dynamics. If so, exceptionally preserved fossil anatomy would reflect a succession of ancient contemporaneous microbial communities, about which we know nothing, rendering decay experiments uninformative. Here, we investigate two questions: (1) what is the role of exogenous and endogenous bacteria during formation of the thanatomicrobiome and (2) do t..., , , # Data from: The relationship between microbial community succession, decay, and anatomical character loss in non-biomineralized animals [https://doi.org/10.5061/dryad.8931zcs07](https://doi.org/10.5061/dryad.8931zcs07) 7 Supplementary data files pertaining to: DNA extraction info and sample info (**1_DNA_extraction_info.xlsx**), Sample Naming conventions, Bacterial Diversity, Sample contamination, and DNA quantity and quality data (**2_OTUreads_QPCRvalues_Alpha_BetaDiversity.xlsx**), Amphioxus decay data (**3_Decay_experiment_data.xlsx**), PCoA analyses (**4_PCoA_data.xlsx**), Operational Taxonomic Unit raw data (**5_OTU_Raw_data.xlsx**), Operational Taxonomic Unit abundance per sample (**6_OTU_abundance_per_sample.xlsx**), Respiratory mode and major electron accepter data for Operational Taxonomic Units (**7_OTU_anerobic_vs_aerobic.xlsx**). ### Data and file structure 7 files organized as: **1_DNA_extraction_info.xlsx**: DNA Extraction information. **1.1. Extraction kit info** (...,以假说为驱动的腐烂实验的一项基本假设为：在腐烂过程中，生物体解剖结构的丧失遵循一套大体由组织内在组成特性所调控的序列。近期针对死后内源微生物群落（thanatomicrobiome）演替的相关研究，对这一假设提出了挑战。这些研究表明，体内的死后微生物组会随着尸体内发生的物理、化学环境变化呈现出可预测的演替过程。因此，腐烂过程中可重复的特征丧失序列，有可能由死后微生物组的演替动态所调控。若该推论成立，那么保存异常完好的化石解剖结构，将反映出一套我们尚一无所知的古代同期微生物群落演替，这会使得腐烂实验的结果失去参考价值。在此，我们探讨两个问题：（1）外源与内源细菌在死后微生物组形成过程中发挥着怎样的作用？（2）…… # 数据源自：《非矿化动物体内微生物群落演替、腐烂过程与解剖特征丧失之间的关联》 [https://doi.org/10.5061/dryad.8931zcs07](https://doi.org/10.5061/dryad.8931zcs07) 本研究附带7份补充数据文件，涵盖内容如下：DNA提取信息与样本信息（**1_DNA_extraction_info.xlsx**）、样本命名规范、细菌多样性、样本污染情况以及DNA数量与质量相关数据（**2_OTUreads_QPCRvalues_Alpha_BetaDiversity.xlsx**）、文昌鱼腐烂实验数据（**3_Decay_experiment_data.xlsx**）、主坐标分析（Principal Coordinates Analysis，简称PCoA）结果（**4_PCoA_data.xlsx**）、操作分类单元（Operational Taxonomic Unit，简称OTU）原始数据（**5_OTU_Raw_data.xlsx**）、每份样本的OTU丰度数据（**6_OTU_abundance_per_sample.xlsx**）、OTU的呼吸模式与主要电子受体相关数据（**7_OTU_anerobic_vs_aerobic.xlsx**）。 ### 数据与文件结构 7份文件的组织形式如下： **1_DNA_extraction_info.xlsx**：DNA提取相关信息。 **1.1. 提取试剂盒信息**（……）