Solanum pimpinellifolium strain:LA1589 Transcriptome or Gene expression

Transcriptome profiling of tomato inflorescence and floral meristems, flowers at 2, 4 and 6 days after floral initiation in wild species S. pimpinellifolium, LA1589 using Illumina RNA-seq. Inflorescence and floral meristems were collected together. Tissues were harvested within one hour (between 10-11 am) and stabilized in RNAlater (Ambion). Replicate 1 and 2 each represents about 100 meristems or flower buds from 5 plants in the field. Replicate 3 and 4 were from a single plant in the greenhouse. Meristems and flower buds were dissected by micro-dissecting forceps under a dissecting microscope. Stand-specific libraries prepared from total RNA and sequences of 51 bp were generated on an Illumina HiSeq2000.