Spiroplasma prevalence in D. hydei following passage under four different thermal regimes

The data represents screening results of F3 flies of <i>D. hydei</i> for <i>Spiroplasma</i> following two generations of passage at different temperatures. Column A: Origin of fly/<i>Spiroplasma</i> isoline. Two levels: Mexico or Cambridge isolines. Column B: Temperature. Temperature during passage over previous two generations. Four levels: 25 °C, 18°C, 18/15°C (diurnal variation), 15 °C. Column C: N of flies tested for <i>Spiroplasma</i>, which had passed QC for DNA template amplifiability using COI prmers. Column D: N of flies scoring as infected on <i>Spiroplasma</i> PCR assay. Column E: Prevalence. Point estimate of proportion flies infected (Column D/Column C) Column F: Upper confidence interval for <i>Spiroplasma</i> prevalence, calculated through binomial sampling. Column G: Lower confidence interval for <i>Spiroplasma</i> prevalence, calculated through binomial sampling.