Dual RNA-seq analysis reveals the interaction between multidrug-resistant Klebsiella pneumoniae and host in a mouse model of pneumonia

Multidrug-resistant Klebsiella pneumoniae (MDR-KP) poses a significant global health threat, associated with high morbidity and mortality rates among hospitalized patients. The interaction between MDR-KP and its host is highly complex. Here, we explored these interactions in a mouse model of pneumonia using dual RNA-seq analysis.Our results revealed that, compared to the low-dose group, the high-dose group significantly upregulated hypoxia and pro-inflammatory cytokine-related genes in the host, and siderophore-related genes in the bacteria. Correlation analysis demonstrated a significant association between siderophore-related genes and clusters of genes related to pro-inflammatory cytokines and hypoxia. Overall design: Mice were infected with either high or low doses of Klebsiella pneumoniae strain NY13307. Dual RNA sequencing analysis of the lung tissues from infected mice was performed at 0 h, 6 h, 12 h, 18 h, and 24 h post-infection, with three mice per group.The lung tissues of healthy mice and the bacterial suspension in PBS were used as control groups for the host and bacteria, respectively.