Miniaturized Filter-Aided Sample Preparation (MICRO-FASP) Method for High Throughput, Ultrasensitive Proteomics Sample Preparation Reveals Proteome Asymmetry in Xenopus laevis Embryos

We report a miniaturized filter aided sample preparation method (micro-FASP) for low-loss preparation of submicrogram proteomic samples. The method employs a filter with ∼0.1 mm2 surface area, reduces the total volume of reagents to本研究报告了一种微型化滤器辅助样本制备方法（微-FASP），该方法适用于低损耗地制备亚微克蛋白质组样本。该技术采用表面面积为∼0.1 mm²的滤器，显著降低了试剂总体积。