WGS of mitonuclear introgression lines of Drosophila melanogaster

In order to study mitonuclear interactions, D. melanogaster populations comprising fully-factorial combinations of mitochondrial and nuclear genomes from Australia, Benin and Canada (A, B, C) respectively) were produced. The crossing scheme was designed to produce distinct mitochondrial backgrounds bearing equivalent pools of standing nuclear variation, by introgressing populations either reciprocally or to themselves. For brevity, we abbreviate population names, giving mitochondrial and then nuclear origin (e.g. AB = Australian mitochondria, Beninese nuclei). Each mitonuclear combination was replicated and whole genomes of two of the replicates were sequenced using a Pool-seq approach.