Mean association and dissociation kinetic rate constants (k_on, k_off) and equilibrium dissociation constants (KD), calculated from surface plasmon resonance (SPR) measurements for aqueous Mini-B (MB) and Super Mini-B (S-MB) flowing past chip-linked Cys-MB and Cys-S-MB monolayer

aMB and S-MB in running buffer (10 mM HEPES, 150 mM NaCl, 3 mM EDTA, 0.005% Surfactant P20, pH 7.4) were flowed past monolayers of N-terminal Cys-MB or N-terminal Cys-S-MB, linked via their respective N-terminal thiol groups to CSM sensor chips in a Biacore 3000 system (Methods). Mean kinetic rate constants (kon, koff) and equilibrium dissociation constants (KD = koff/kon) were determined from curve fitting analyses of SPR results at 1 µg peptide/ml buffer.