Transcriptome-wide effects of NusA on RNA polymerase pausing in Bacillus subtilis. Transcriptome-wide effects of NusA on RNA polymerase pausing in Bacillus subtilis
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In this work we conducted RNET-seq on Wild Type, NusA depletion, nusG deletion, and NusA depletion nusG deletion strains of B. subtilis. Using this data we analysed the transcriptome-wide effects of NusA on RNA polymerase pausing and found that NusA has modest pause stimulating as well as suppressing effects throughout the genome. Overall design: RNET-seq on Wild Type, NusA depletion, nusG deletion, and NusA depletion nusG deletion strains of B. subtilis followed by in vitro biochemistry follow-up.
创建时间:
2021-10-20



