Proteomic characterization of <i>Pseudogymnoascus</i> spp. isolates from polar and temperate regions

Proteomics has been used extensively in the field of mycology, mainly in trying to understand the complex network of protein-protein interactions that has been implicated in the molecular functions of fungi. It is also a useful tool to compare metabolic differences within a genus. Species of <i>Pseudogymnoascus</i>, a genus under the phyla Ascomycota, have been shown to play an important role in the soil environment. They have been found in both polar and temperate regions and are a known producer of many extracellular hydrolases that contribute to soil decomposition. Despite the apparent importance of <i>Pseudogymnoascus</i> spp. in the soil ecosystem, investigations into their molecular functions are still very limited. In the present study, proteomic characterization of six <i>Pseudogymnoascus</i> spp. isolated from three biogeographic regions (the Arctic, Antarctic, and temperate regions) was carried out using tandem mass spectrometry. Prior to proteomic analysis, the optimization for protein extraction was carried out. Trichloroacetic acid‑acetone‑phenol was found to be the best extraction method to be used for proteomic profiling of <i>Pseudogymnoascus</i> spp. The proteomic analysis identified 2003 proteins that were successfully mapped to the UniProtKB database. The identified proteins were clustered according to their biological processes and molecular functions. The shared proteins found in all <i>Pseudogymnoascus</i> spp. (1201 proteins) showed a significantly close relationship in their basic cellular functions, despite differences in morphological structures. Analysis of <i>Pseudogymnoascus</i> spp. proteome also identified proteins that were unique to each region. However, a high number of these proteins belonged to protein families of similar molecular functions, namely, transferases and hydrolases. Our proteomic data can be used as a reference for <i>Pseudogymnoascus</i> spp. across different global regions and a foundation for future soil ecosystem function research.

蛋白质组学（Proteomics）已在真菌学（mycology）领域得到广泛应用，主要用于解析与真菌分子功能密切相关的复杂蛋白质-蛋白质相互作用网络。同时，该技术也是比较同一属内真菌代谢差异的有效工具。子囊菌门（Ascomycota）下的假裸囊菌属（*Pseudogymnoascus*）真菌，已被证实对土壤环境具有重要作用。该属真菌广泛分布于极地与温带地区，且已知可分泌多种参与土壤有机质分解的胞外水解酶（hydrolase）。尽管假裸囊菌属物种（*Pseudogymnoascus* spp.）在土壤生态系统中具有显著重要性，但目前针对其分子功能的研究仍十分有限。本研究采用串联质谱（tandem mass spectrometry）技术，对分离自北极、南极与温带三大生物地理区域的6株假裸囊菌属物种进行了蛋白质组学表征分析。在正式开展蛋白质组学分析前，本研究先对蛋白质提取方法进行了优化。实验结果显示，三氯乙酸-丙酮-苯酚法是最适合假裸囊菌属物种蛋白质组学分析的提取方法。本次蛋白质组学分析共鉴定出2003个可成功比对至通用蛋白质知识库（UniProtKB）的蛋白质。研究人员根据这些鉴定得到的蛋白质的生物学过程与分子功能，对其进行了聚类分析。所有假裸囊菌属物种共有的1201个核心蛋白质，尽管各菌株间存在形态结构差异，但其基础细胞功能的关联度显著较高。对假裸囊菌属物种蛋白质组的分析还鉴定出了各生物地理区域特有的蛋白质。但其中多数蛋白质均属于功能相似的蛋白质家族，即转移酶（transferase）与水解酶。本研究产生的蛋白质组学数据，可作为全球不同区域假裸囊菌属物种研究的参考数据集，同时也可为未来土壤生态系统功能相关研究奠定基础。

搜集汇总

背景与挑战

背景概述

该数据集是一个蛋白质组学数据集，专注于对来自北极、南极和温带地区的六个Pseudogymnoascus属真菌分离株进行蛋白质特征分析。研究使用串联质谱法鉴定了2003个蛋白质，并优化了蛋白质提取方法（三氯乙酸-丙酮-酚法），发现所有分离株共享1201个蛋白质，表明它们在基本细胞功能上密切相关，同时每个区域有独特的蛋白质，主要属于转移酶和水解酶家族。